The inhibitory effect of aspisol on human breast cancer cells in vitro and its mechanism
10.3781/j.issn.1000-7431.2008.07.005
- Author:
Yue-Lin ZHANG
1
Author Information
1. Department of Pharmacology
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Cyclooxygenase2 inhibitors;
Gene expression;
Matrix metalloproteinases
- From:
Tumor
2008;28(7):563-566
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of aspisol on expressions of cyclooxygenese-2 (COX-2), matrix metalloproteinaseses-9 (MMP-9) proteins in human breast cancer MDA-MB-231 cells. Methods: MDA-MB-231 cells were incubated with aspisol at various concentrations for 24 h. The morphological changes of MDA-MB-231 cells were evaluated by HE staining. The inhibitory effects of aspisol on the proliferation of MDA-MB-231 cells were determined by MTT assay. The effects of aspisol on the expressions of COX-2 and MMP-9 in MDA-MB-231 cells were measured by Western blotting. Results: Under light microscope the cells in aspisol treatment groups showed decreased density, round shape, and light staining of nucleus. Aspisol significantly inhibited the proliferation of MDA-MB-231 cells (P < 0.01). Compared with the control group, aspisol dose-dependently inhibited the expressions of COX-2 and MMP-9 protein in MDA-MB-231 cells (P < 0.05). Conclusion: This study suggested that aspisol inhibited the expressions of COX-2 and MMP-9 protein in breast cancer MDA-MB-231 cells.
