Alteration of the methylation status of RUNX 3 gene in bladder cancer
10.3781/j.issn.1000-7431.2008.08.016
- Author:
Na YANG
1
Author Information
1. Department of Clinical Laboratory Medicine
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Gene, RUNX 3;
Methylation;
Methylation specific PCR
- From:
Tumor
2008;28(8):702-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the methylation status of human runt-related transcription factor 3 (RUNX 3) gene in bladder cancer tissues and investigate its role in tumorigenesis and progression of bladder caner. Methods: Methylation-specific PCR (MSP) was used to examine the methylation status of RUNX 3 gene in 33 cases of bladder cancer tissues, 8 cases of paired adjacent normal tissues, and 3 cases of normal bladder tissues from non-tumor patients. Results: Aberrant methylation of RUNX 3 was found in 21 out of 33 (63.7%) bladder cancer tissues and 1 out of 8 (12.5%) tumor adjacent normal tissues. There was statistical significance between them (P <0.05). Three normal bladder tissues from non-tumor patients showed no abnormal methylation of RUNX 3. Conclusion: Hypermethylation of RUNX 3 gene in bladder cancer tissues suggests that aberrant methylation of RUNX 3 correlates with tumorigenesis of bladder cancer.
