Expression of survivin in endometrial cancer and its role in regulating apoptosis of endometrial cancer cells
10.3781/j.issn.1000-7431.2008.08.012
- Author:
Zhi-Hong AI
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Endometrial neoplasms;
Gene silencing;
Inhibitor of apoptosis proteins;
Survivin
- From:
Tumor
2008;28(8):685-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the expression of survivin in endometrial cancer and its roles in regulating apoptosis of endometrial cancer cells. Methods: Imnumohistochemistry was used to examine the expression of survivin in endometrial cancer specimens. RNA interference (RNAi) was applied to knock down survivin expression. The effect of RNAi was examined by RT-PCR and Western blotting. Cell apoptosis was detected by the FCM before and after RNAi. Western blotting was used to detect the expressions of caspase-3, caspase-8 and Bcl-2, which were closely associated with apoptosis. Results: The positive rate of survivin protein in endometrial cancer was obviously higher than those in atypical endometrium and normal endometrium (P < 0.05 and P < 0.01, respectively). RNAi effectively inhibited the expressions of survivin mRNA and survivin protein (P < 0.01). RNAi also induced cell apoptosis. Cell apoptosis rates in the two groups of RNAi were obviously higher than those in control groups (P < 0.01). Survivin RNAi also up-regulated the cleavage of caspase-3 and caspase-8. Conclusion: The aberrant expression of survivin may be closely related to the carcinogenesis of endometrial cancer, and inhibition of its expression may induce apoptosis of endometrial cancer cells.
