Effect of macrophages with different activated phenotypes on tumor growth, lymphangiogenesis and lymph node metastasis in mice bearing Lewis lung carcinoma
10.3781/j.issn.1000-7431.2008.08.006
- Author:
Bi-Cheng ZHANG
1
Author Information
1. Department of Oncology
- Publication Type:Journal Article
- Keywords:
Animal experimentation;
Carcinoma, Lewis lung;
Macrophage activation
- From:
Tumor
2008;28(8):660-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of macrophages with different activated phenotypes on tumor growth, lymphangiogenesis and lymp node metastasis in mice bearing Lewis lung carcinoma (LLC). Methods: The xenografted models were established via subcutaneous injection of LLC cells and activated macrophages into mice. They were divided into 4 groups: blank control group, classically activated macrophages (caMphi) group, alternatively activated macrophages (aaMphi) group and RAW264. 7 group with 10 mice in each group. The growth of transplanted tumors was examined dynamically and then the mice were sacrificed on d 28. The transplanted tumors were resected and the lymph nodes and distant metastasis were observed by HE staining. The LYVE-1+ lymphatic vessel densities (LVD) were detected by immunohistochemistry. Furthermore, other 40 mice were randomly divided into the four groups and the survival rates were recorded. Results: Compared with the blank control group, the aaMphi and RAW264. 7 xenografted tumors grew faster and had more lymph nodes and lung metastasis (P < 0.01) as well as increased LVD and decreased survival rates (P < 0.05) in the aaMphi group and RAW264.7 group. The number of metastatic lymph nodes and LVD were significantly higher in the aaMphi group than those in the RAW264. 7 group (P < 0.05). However, there was no significant difference in tumor growth, lung metastatic nodes and survival rate of tumor-bearing mice between the two groups (P > 0.05). Conclusion: aaMphi can promote the growth, lymphangiogenesis, lymph nodes and lung metastasis in transplanted LLC tumors, and reduce the survival rate of tumor-bearing mice. In the tumorigenesis of transplanted LLC tumors, RAW264. 7 cells may toward aaMphi.
