Id2 promotes migration of SKOV3 ovarian cancer cells by non-HLH domain
10.3781/j.issn.1000-7431.2008.09.012
- Author:
Cheng-Lei GU
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- Keywords:
Cadherins;
Inhibitors of differentiation;
Neoplasm invasiveness;
Ovarian neoplasms
- From:
Tumor
2008;28(9):767-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the influence of inhibitor of differentiation 2 (Id2) on cell proliferation and migration in SKOV3 cells, and to investigate the influence of HLH domain deletion in 1d2 gene on SKOV3 cells. Methods: Human ovarian cancer cell line SKOV3 was transfected with pcDNA3.1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors by SuperFect Transfection Reagent. The cells transfected with blank vector pCDNA3. 1 were used as control. Id2 protein was detected by Western blot, and mRNA transcriptions of Id2, Id2-DBM (D-box mutant), and Id2-DBM-δHLH were measured by RT-PCR. Cell proliferation was assessed by MTT assay. Cell invasion was detected by scratch test and transwell chamber assay. The regulatory effect of Id2 on E-cadherin was evaluated by Western blot. Results: The mRNA and protein levels indicated that the plasmid was successfully transfected. Cell growth curve suggested that the proliferation of cells had no significant difference between each group. Compared with the control group, cell migration ability was significantly enhanced after transfection with pcDNA3. 1-Id2, pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors. The downregulation of E-cadherin protein was accompanied with increased migration capability in pcDNA3. 1-Id2-DBM and pcDNA3. 1-Id2-DBM-δHLH vectors-transfected cells. Conclusion: Overexpression of Id2 promotes the migration capability of SKOV3 cells, which might be related with the downregulation of E-cadherin. The action still exists after the HLH domain deletion in Id2 gene.
