The effects of ANGPTL4 gene on the growth of different HCC cell lines
10.3781/j.issn.1000-7431.2008.10.001
- Author:
Yi-Yuan ZENG
1
Author Information
1. Shanghai Medical College
- Publication Type:Journal Article
- Keywords:
Angiopoietin-like 4;
Cell growth;
Experimental;
Liver neoplasms;
Tumor cells, culture
- From:
Tumor
2008;28(10):819-824
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of angiopoietin-like 4 (ANGPTL4) gene on the growth of different hepatocellular carcinoma (HCC) cell lines. Methods: The expression of ANGPTL4 was tested by reverse transcriptase-polymerase chain reaction and Western blotting in 9 HCC cell lines and immortal liver cells. Huh-7 cells and MHCC-LM3 cells with low ANGPTL4 expression and MHCC-97L cells with moderate ANGPTL4 expression were transfected with fused ANGPTL4-GFP and empty pEGFP-N1 plasmids via Lipofetamine 2000, respectively. GFP-positive cells were screened with G418 and sorted by flow cytometry (FCM) to establish stable cell lines with over-expression of ANGPTL4, Cell array was prepared and Ki-67 fluorescent immunocytochemical techniques were used to detect in vitro proliferation of the three HCC cell lines. Xenografted tumor formation in nude mice was used to observe the effect of ANGPTL4 on the growth of the three HCC cells in vivo. Results: ANGPTL4 mRNA had low expression in Huh-7 and MHCC-LM3 cell lines, moderate expression in MHCC-97L cells, and high expression in Hep3B cells. The results of cell array showed that cell proliferation rate was lower in Huh-7 cells (P = 0.000 74), but higher in MHCC-LM3 (P = 0.073 08) and MHCC-97L (P = 0.011 52) cells with over-expression of ANGPTL4 compared with the cells transfected with empty plasmid. Over-expression of ANGPTL4 significantly inhibited xenografted tumor formation of Huh-7 cells (P = 0.001 10), but markedly promoted the xenografted tumor formation of MHCC-LM3 (P=0.057 50) and MHCC-97L (P = 0.018 91) cells in nude mice. Conclusion: ANGPTL4 plays different roles in the growth of different HCC cell lines, but the effects of ANGPTL4 on the growth of the same cell line was consistent in vitro and in vivo.
