Application of radionuclide bone imaging in establishment of a human lung adenocarcinoma cell line SPC-A-1BM
10.3781/j.issn.1000-7431.2008.11.006
- Author:
Shun-fang YANG
1
Author Information
1. Department of Nuclear Medicine
- Publication Type:Journal Article
- Keywords:
Adenocarcinoma;
Lung neoplasms;
Mice, nude;
Neoplasm metastasis;
Radionuclide imaging
- From:
Tumor
2008;28(11):933-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The purpose of this study was to establish a human lung adenocarcinoma cell line SPC-A-1 BM with high bone metastatic potential and detect the bone metastasis in immunodeficient NIH-BNX mice by using in vivo radionuclide bone imaging technology. Methods: The human lung adenocarcinoma cell line SPC-A-1 was injected into left ventricle of mice to induce bone metastasis. The metastatic lesions in mice were traced by radionuclide bone imaging agent. The lesion was resected and the isolated tumor cells were cultured in vitro to obtain the bone metastatic human lung adenocarcinoma cells. The first passage of bone metastatic human lung adenocarcinoma cells were injected again into mice via vascular system (artery and vein) or transplanted in lung orthotopically. The cells were repeatedly screened for several times. Results: Chromosome analysis showed that the bone metastatic SPC-A-1BM cells detected by radionuclide bone imaging still kept the properties of human origin after several cycles of screening in vivo and in vitro. The influence of 99m Tc-MDP (111 MBq) on the growth of SPC-A-1BM cells was similar with or a little less than X ray film (40 kV, 2 mA, 4 s). The overall sensitivity, specificity and accuracy of radionuclide bone imaging and X-ray film were 97.6%, 73.3%, 94% and 31.3%, 100%, 43%, respectively. Conclusion: The radionuclide bone imaging offers a practical and convenient method for establishing a human lung adenocarcinoma cell line SPC-A-1BM with high bone metastatic potential and detecting the bone metastasis in immunodeficient BNX mice.
