Screening differential protein expressions in colorectal carcinoma cell lines with varying metastatic potentials using proteomic analysis method
10.3781/j.issn.1000-7431.2008.11.004
- Author:
Long HUANG
1
Author Information
1. Department of Surgery
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Electrophoresis;
Gel;
Proteomics;
Two-dimensional
- From:
Tumor
2008;28(11):925-928
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the differential protein expressions in highly-metastatic colorectal carcinoma cell lines LoVo and low-metastatic SW480 cells using proteomic analysis method and detect the biological molecule for early diagnosis of metastasis of colo-rectal carcinoma. Methods: Proteins in highly-metastatic LoVo cells and low-metastatic SW480 cells were isolated by two-dimension gel electrophoresis (Two -DE). Two-DE map was established by gel-silver staining and Coomassie brilliant blue staining. The well differentially expressed protein spots were screened by Image Master imaging. The specific protein spots were identified by peptide mass fingerprints using matrix-assisted laser desorption/time of flight mass spectrometry (MALDI-TOF/MS) and through searching NCBInr database using Mascot software. Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells. Conclusion: This study has found that the differential proteins are related with tumor growth, migration, adhesion and apoptosis. Our results provide theoretical basis for finding biological markers for early diagnosis of metastasis of colorectal carcinoma.
