Three-dimensional culture of the primary lung adenocarcinoma tissues
10.3781/j.issn.1000-7431.2008.12.018
- Author:
Heng ZHANG
1
Author Information
1. Department of Thoracic Surgery
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Three-dimentional culture;
Tissue culture techniques
- From:
Tumor
2008;28(12):1095-1098
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the three-dimensional (3-D) culture technique of the lung adenocarcinoma tissues in vitro and further evaluate its application foreground. Methods: In order to mimick the tumor growth in vivo, primary lung cancer tissues were cultured in well-prepared 3-D collagen-based gel in vitro. Optical microscopy, electron microscopy, immunohistochemistry and tumor transplantation techniques were uesd to observe the growth of the tumor tissues. Results: Primary lung adenocarcinoma tissues were grown in collagen mixed with fibroblasts in 3-D space. After the culture period was prolonged, the volume of tumor tissues turned small. Connection was established among adjacent tumor tissues by migration of tumor cells. Some tumor cells permeated the gel, migrated to the bottom of the culture plate, and grew on it. Immunohistochemistry analysis indicated the well growth of tumor tissues. Conclusion: Primary lung adenocarcinoma tissues grow well in 3-D culture enviroment and keep the invasion features. The 3-D culture technique supplies a research platform for mimicking the real enviroment of tumor in vivo.
