Preparation of monoclonal antibody against GPR48 and its expression in human colorectal cancers
10.3781/j.issn.1000-7431.2008.12.007
- Author:
Yun GAO
1
Author Information
1. Endocrinology Institute
- Publication Type:Journal Article
- Keywords:
Antibody, monoclonal;
Colorectal neoplasms;
Immunohistochemistry
- From:
Tumor
2008;28(12):1047-1050
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare the monoclonal antibody of G protein coupled receptor 48 (GPR48) and investigate the expression in colorectal cancers. Methods: The monoclonal antibody of GPR48 was produced by cell fusion technology and identified by Western blotting and flow cytometry (FCM). The expression of GPR48 was studied in colorectal cancer by using the immunohistochemical method. Results: The specific monoclonal antibody of GPR48 was prepared. No expression of GPR48 was detected in normal colorectal tissues. The positive rate of GPR48 was significantly higher in colorectal cancer patients with lymph node metastasis than that in patients without lymph node metastasis (85.7% vs 34.6% P <0.05); it was significantly lower in well-differentiated colorectal cancer than that in moderately- and poorly-differentiated colorectal cancer (22.2% vs 77.3%, P <0.05). GPR48 expression had no correlation with the sex and tumor size of patients. Conclusion: The monoclonal antibody of GPR48 is a specific antibody. It can be used as a new index for predicting tumor differentiation and lymph node metastasis.
