Effects of ginsenoside Rg3 on the invasion and metastasis of mouse melanoma cell line B16 as well as the expression of MMP-9
10.3781/j.issn.1000-7431.2011.02.005
- Author:
Xin JIANG
1
Author Information
1. Department of Radiotherapy
- Publication Type:Journal Article
- Keywords:
Ginsenosides;
Matrix metalloproteinase;
Melanoma, experimental;
Neoplasm invasiveness;
Neoplasm metastasis
- From:
Tumor
2011;31(2):117-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effects of ginsenoside Rg3 on the invasive and metastatic potentials of mouse melanomas B16 cells in vitro and in vivo, as well as the expression of matrix metalloproteinase-9 (MMP-9), and to investigate the underlying mechanisms. Methods: The grafted melanoma and its spontaneous lung metastasis model in mice was established by transplanting with B16 cells. The number of metastatic lesions in the lung was calculated, and the expression of MMP-9 in melanoma tissues was detected by immunohistochemistry, after intraperitoneal injection of ginsenoside Rg3 or 0.9% NaCl solution (as the control). The invasive ability of B16 cells treated with ginsenoside Rg3 was detected by invasion assay, and the expression of MMP-9 was evaluated by immunocytochemistry in vitro. Results: Compared with the control group, the numbers of metastatic lesions in the lung were all decreased in three ginsenoside Rg3 (0.3, 1.0 and 3.0 mg/kg)-treated groups, and the expression levels of MMP-9 in tumor tissues were also decreased in these three groups ( P<0.05). The numbers of B16 cells invading through artificial basal membrane were both fewer in ginsenoside Rg3 (2.5 and 5.0 μg/mL)-treated groups than those in the control group ( P<0.01), in vitro. Moreover, 5.0 μg/mL ginseno-side Rg3 inhibited the expression level of MMP-9 in B16 cells. Conclusion: Ginsenoside Rg3 can obviously inhibit the lung metastasis of melanoma in mice, and this inhibitory effect may be due to suppressing the invasive ability of B16 cells as well as the expression level of MMP-9.
