Expression of histidine triad nucleotide-binding protein 1 in clear cell renal cell carcinoma and its clinical significance
10.3781/j.issn.1000-7431.2011.05.011
- Author:
Bin YANG
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Gene expression;
Histidine triad nucleotide-binding protein 1;
Immunohistochemistry;
Kidney neoplasms;
Reverse transcriptase polymerase chain reaction
- From:
Tumor
2011;31(5):436-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the expressions of histidine triad nucleotide-binding protein 1 (HINT1) mRNA and protein in clear cell renal cell carcinoma (ccRCC) and their relationships with clinicopatholgical features. Methods: Real-time fluorescence quantitative reverse transcription PCR (RFQ-RT-PCR) was used to detect the expressions of HINT1 mRNA in 36 cases of fresh ccRCC and 37 cases of normal kidney tissues (29 paired cases), as well as their relationships with clinicopathological features were analyzed. Immunohistochemistry was used to detect the distribution and expression of HINT1 protein in 30 cases of paraffin embedded fresh ccRCC tissues and 12 cases of paraffin-embedded adjacent normal kidney tissues, as well as their relationships with clinicopathological features were analyzed. Results: The expression levels of HINT1 mRNA in 29 cases of paired ccRCC and normal kidney tissues were 0.209±0.033 and 0.733±0.136, respectively (P<0.001). The expression levels of HINT1 mRNA in 36 cases of ccRCC and 37 cases of normal kidney tissues were 0.245±0.035 and 0.694± 0.108, respectively (P<0.001). The positive expression rate of HINT1 protein in the adjacent normal kidney tissues was 100% (12/12), diffusely distributing in the nucleus and cytoplasm. HINT1 protein immunostaining in proximal convoluted tubule was stronger than that in distal convoluted tubule, while it was partially weaker in glomerular basement membrane and Bowman's capsule. The HINT1 protein expression was negative in renal interstitium. HINT1 protein expression distributing in nucleus and cytoplasm was 60% (18/30) positive in ccRCC, while it was negative in intercellular substance (P<0.01). The tendency of HINT1 mRNA expression in ccRCC and normal kidney tissues and their relationships with the clinicopathological features were the same as those of HINT1 protein. The expressions of HINT1 mRNA and protein in stages T1-2 of ccRCC were higher than those in stages T3-4 (P<0.05), and the same result was found in normal kidney tissues and ccRCC tissues. The expressions of HINT1 mRNA and protein were not correlated with Fuhrman grade, gender, age and tumor size (P>0.05). Conclusion: HINT1 may play a role as tumor suppressor gene in ccRCC. The abnormal expression of HINT1 may be associated with the abnormal regulation before translation. Abnormal expressions of HINT1 mRNA and protein may serve as a marker for the prognosis of ccRCC patients.
