Effects of vector-mediated targeting human telomerase reverse transcriptase gene RNA interference on proliferation and apoptosis of leukemia HL-60 cells
10.3781/j.issn.1000-7431.2011.05.008
- Author:
Ji-Zhao GAO
1
Author Information
1. Department of Pediatrics
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell proliferation;
Cell, HL-60;
Leukemia, experimental;
RNA interference;
Telomerase reverse transcriptase
- From:
Tumor
2011;31(5):424-427
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of vector-mediated targeting human telomerase reverse transcriptase ( hTERT) gene RNA interference (RNAi) on the proliferation and apoptosis of leukemia HL-60 cells. Methods: The targeting hTERT gene RNAi recombination vector (pSilencer1.0-U6/hTERT) was transfected into HL-60 cells. The HL-60 cells transfected with empty vector (pSilencer1.0-U6), RNAi-mate or HL-60 cells were used as the controls. The expression of hTERT protein was detected by Western blotting. The proliferation and apoptosis of HL-60 cells were measured by MTT assay and flow cytometry, respectively. Results: The expression levels of hTERT protein in HL-60 cells were lower and the proliferation inhibitory rate and apoptosis rate of HL-60 cells were higher than those of each control group after transfection with pSilencer1.0-U6/hTERT for 24, 72, and 120 h ( P<0.05). There was no differences among HL-60 cells transfected with pSilencer1.0-U6/hTERT for 24, 72, and 120 h, and no differences were also observed among the three control groups ( P>0.05). Conclusion: Vector-mediated targeting hTERT gene RNAi can down-regulate the expression of hTERT protein and inhibit the proliferation and induce apoptosis of HL-60 cells in vitro.
