Evodiamine combined with radiation inhibits proliferation, adhesion and migration of human tongue squamous cell carcinoma cell line Tca-8113
10.3781/j.issn.1000-7431.2011.05.007
- Author:
Rui FENG
1
Author Information
1. Department of Restorative Dentistry
- Publication Type:Journal Article
- Keywords:
Cell adhesion;
Cell migration assays;
Cell proliferation;
Evodiamine;
Radiotherapy;
Tongue neoplasms
- From:
Tumor
2011;31(5):417-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of evodiamine (EVO) combined with radiation on radiosensitivity and adhesion as well as migration abilities of human tongue squamous-cell cancer Tca-8113 cells. Methods: Cell proliferation inhibitory rates of Tca-8113 cells were detected by MTT method at different time points after treatment with EVO at different concentrations. The colony-formation ability of Tca-8113 cells treated with EVO plus radiation was detected by colony-formation assay. The changes of adhesion and migration abilities of Tca-8113 cells were examined by using cell adhesion assay and wound healing assay, respectively. Results: Survival rate of Tca-8113 cells decreased in association with the elevation of EVO concentration and longer treatment time. However, the survival rates of Tca-8113 cells at different time points after treatment with EVO at different concentrations in the EVO plus radiation group were significantly lower than those in the radiation group ( P<0.01). The calculated values of sensitizing enhancement ratio (SER) Do, SERDq and SERSF2 through clony-forming assay were 1.35, 1.75 and 1.67, respectively. The homotypic adhesion test revealed that the numbers of adhering cells in the EVO plus radiation group and the radiation group were significantly higher than that in the control group at 20, 40 and 60 min after vibration ( P<0.01). The number of adhering cells in EVO plus radiation group was significantly higher than that in the radiation group at 40 and 60 min after vibration ( P<0.01). The endothelial cell adhesion assay indicated that the adhesion rates of Tca-8113 cells in the EVO plus radiation group obviously decreased compared with those in the radiation group and the control group at 20, 40 and 60 min after vibration ( P<0.05). The wound healing assay revealed that the migration rate of Tca-8113 cells in EVO plus radiation group obviously decreased compared with those in the radiation group and the control group (<0.01). Conclusion: Evodiamine can significantly enhance the radiosensitivity of human tongue cancer Tca-8113 cells, and can change the adherence and migration capacities of Tca-8113 cells.
