RNA interference targeting CXCR7 gene inhibits proliferation and migration of colon cancer cell line SW1116
10.3781/j.issn.1000-7431.2011.05.006
- Author:
Ming-Yuan QIU
1
Author Information
1. Department of General Surgery
- Publication Type:Journal Article
- Keywords:
Cell migration;
Cell proliferation;
Chemokine receptor 7;
Colorectal neoplasms;
RNA, small interfering
- From:
Tumor
2011;31(5):412-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of chemokine receptor 7 (CXCR 7) gene on the proliferation and migration of human colon cancer cell line SW1116. Methods: After transfection with small interfering RNA (siRNA) targeting CXCR 7, the expressions of CXCR7 mRNA and protein in human colon cancer cell line SW1116 were determined by RT-PCR and Western blotting, respectively. The effects of altered expression of CXCR7 on cell proliferation and migration were measured by CCK-8 cell proliferation assay and Transwell migration assay, respectively. Results: At 24-hour post-transfection, the relative expression level of CXCR7 mRNA in the CXCR7-siRNA transfection group (0.275±0.018) was reduced by (56.6± 3.8)% as compared with that of the blank control group (0.635±0.024). At 48-hours post-transfection, the relative expression CXCR7 protein in the CXCR7-siRNA transfection group was significantly decreased than those in the negative control group and blank control group (P<0.05). The proliferation abilities of SW1116 cells at 48-, 72-, 96- and 120-hour after CXCR7-siRNA transfection were significantly decreased than those in the negative control group and blank control group (P<0.05). The number of SW1116 cells migrating across the polycarbonate membrane in the CXCR7-siRNA transfection group (22.73±2.01) was significantly lower than those in the negative control group (49.20±3.82) and blank control group (54.80± 4.85) (P<0.05). Conclusion: siRNA targeting CXCR 7 gene can inhibit the proliferation and migration of human colon cancer cell line SW1116.
