Effect of adenovirus-associated virus carrying kringle 1 domain of hepatocyte growth factor gene on the growth of human prostatic carcinoma xenograft in nude mouse bone
10.3781/j.issn.1000-7431.2011.07.003
- Author:
Zeng-Jun LIU
1
Author Information
1. Department of Oncology, Sixth People's Hospital, Shanghai Jiaotong University
- Publication Type:Journal Article
- Keywords:
Disease models, animal;
Kringle 1 domain of hepatocyte growth factor;
Neovascularization, pathologic;
Prostatic neoplasms
- From:
Tumor
2011;31(7):585-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of adenovirus-associated virus carrying kringle 1 domain of hepatocyte growth factor (HGFK 1) gene on the growth of human prostatic carcinoma xenograft in nude mouse bone. Methods: The human prostatic carcinoma xenograft in nude mouse bone was established by injection of PC-3 cells into the mouse tibial bone marrow cavity. PBS or the supernatant of recombinant adenovirus-associated virus carrying enhanced green fluorescent protein (EGFP ) gene (rAAV-EGFP) or HGFK 1 gene (rAAV-HGFK1) was injected into the prostatic carcinoma bone xenograft or into the tail vein. Tumor growth, metastasis and the survival of nude mice were observed. The expression levels of Ki67, E-cadherin and CD31 in the bone xenograft were detected by immunohistochemistry. Results: The human prostatic carcinoma xenograft in nude mouse bone was successfully established. The tumor volume was smaller and the tumor weight was lower in the rAAV-HGFK1 group than those in the PBS group and the rAAV-EGFP group (P <0.05). The tumor growth inhibition rate in the rAAV-HGFK1 group was 46.69%. The median survival of nude mice in the rAAV-HGFK1 group was longer than those in the PBS group and the rAAV-EGFP group (P <0.05). The paraaortic lymph node metastasis rates in the PBS group and the rAAVEGFP group were higher than that in the rAAV-HGFK1 group (P <0.05). The expression levels of Ki67 and CD31 were lower and the expression level of E-cadherin was higher in the rAAV-HGFK1 group than those in the PBS group and the rAAV-EGFP group (P <0.05). Conclusion: HGFK1 can inhibite the growth, metastasis and angiogenesis of human prostatic carcinoma bone xenograft in nude mice, as well as prolong the survival of nude mice. Copyright© 2011 by TUMOR.
