The expression levels of Reg4 and Ki67 in superficial elevated mucosa lesions of atrophic gastritis with intestinal metaplasia are higher than those in superficial depressed mucosa lesions
10.3781/j.issn.1000-7431.2011.10.011
- Author:
Yan-Jie YU
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
Endoscopy;
Gastritis, atrophic;
Intestinal metaplasia;
Precancerous conditions
- From:
Tumor
2011;31(10):937-941
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To infer which morphologic change of gastric mucosa has more probability to develop into dysplasia or cancer by detecting the expressions of regenerating gene 4 (Reg 4) and Ki67 in different morphologies of intestinal metaplasia in gastric antrum. Methods: Immunohistochemistry and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were used to examine the expression levels of Reg4 and Ki67 proteins and mRNAs in mucosal biopsy specimens of 385 cases of different types of gastric disease. Results: In the gastric mucosal biopsy specimens each from nonatrophic gastritis (NAG), atrophic gastritis (AG), atrophic gastritis with intestinal metaplasia displaying superficial depressed mucosa lesions (DIM), atrophic gastritis with intestinal metaplasia displaying superficial elevated mucosa lesions (EIM) and gastric cancer (GC), the positive rates of Reg4 protein was 12.3%, 26.7%, 43.6%, 93.6% and 31.5%, respectively; the positive rate of Reg4 protein of EIM was significantly different from those of NAG, AG, DIM and GC (P <0.05). The positive rates of Ki67 protein were 14.8%,18.7%, 23.1%, 69.2% and 71.2% in NAG, AG, DIM, EIM and GC, respectively; the positive rate of Ki67 protein in EIM was significantly different from those of NAG, AG and DIM (P <0.05) but not different from that of GC (P >0.05). The expressions of Reg4 and Ki67 mRNAs in EIM were also significantly higher than those in DIM. Conclusion: The expression levels of Reg4 and Ki67 in EIM were higher than those in DIM, which suggests that EIM may have more probability to develop into dysplasia or cancer than DIM, and DIMmay become a more valuable specimen for gastric biopsy. Copyright© 2011 by TUMOR.
