The downregulation of HDAC2 expression in esophageal squamous cell carcinoma and its effect on biological features of EC9706 cells
10.3781/j.issn.1000-7431.2011.10.003
- Author:
Na ZHANG
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell proliferation;
Esophageal neoplasms;
Histone deacetylases;
RNA, small interfering
- From:
Tumor
2011;31(10):886-892
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of histone deacetylase 2 (HDAC2) in esophageal squamous cell carcinoma (ESCC), and to explore the effects of downregulation of HDAC2 expression on cell proliferation, cell cycle and apoptosis of EC9706 cells and their possible molecular mechanisms. Methods: The expression of HDAC2 protein in ESCC tissues was detected by immunohistochemistry. HDAC2 small interfering RNA (siRNA) and control siRNA were transfected into ESCC EC9706 cells, and then the EC9706 cells were randomly divided into three groups, including untreated group, siRNA control group and HDAC2 siRNA group. The expression of HDAC2 protein in EC9706 cells was detected by Western blotting. The cell proliferation status was examined by cell counting Kit-8 (CCK-8) method. The cell cycle and apoptosis were detected by flow cytometry (FCM). The expressions of proteins related to cell proliferation, cell cycle and apoptosis were detected by Western blotting. Results: The positive expression rate of HDAC2 in ESCC tissues (79.71%) was significantly higher than those in normal esophageal mucosa (51.11%) and paracancerous tissues (dysplasia, 23.19%), and there was a significant difference among these three tissues (χ2=44.121, P=0.000). The expression of HDAC2 protein wasnot associated with age and gender of patients with ESCC (both P>0.05), but closely associated withhistological grade, invasive depth, TNM stage and lymph node metastasis (all P<0.05). HDAC2 siRNA effectively down-regulated the expression of HDAC2 protein, obviously inhibited the cell proliferation, arrested cell cycle at G0/G1 phase and induced apoptosis of EC9706 cells. In addition, the result of Western blotting showed that down-regulation of HDAC2 expression could markedly increase the expressions of p21 protein and apoptosis-related bax protein while decrease the expressions of cyclin D1 and bcl-2 proteins. Conclusion: HDAC2 may play an important role in occurrence and development of ESCC. The downregulation of HDAC2 expression-mediated proliferation inhibition, cell cycle arrest and apoptosis of EC9706 cells may be tightly associated with the increased expression levels of p21 and baxas well as the decreased expression levels of cyclin D1 and bcl-2. Copyright© 2011 by TUMOR.
