Regenerating gene IV and its carbohydrate-recognition domain promote angiogenesis of colorectal carcinoma xenografts in nude mice
10.3781/j.issn.1000-7431.2011.11.006
- Author:
Ying GUO
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Cell, LoVo;
Colorectal neoplasms;
Mice, nude;
Regenerating gene IV;
Vascular endothelial growth factor
- From:
Tumor
2011;31(11):993-998
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of regenerating gene IV (Reg IV) and its carbohydrate-recognition domain (CRD)on angiogenesis of colorectal carcinoma xenografts in nude mice. Methods: The LoVo human colorectal carcinoma cells were transfected with recombinant plasmids pcDNA3.1-Reg IV (LoVo/RegIV group), pcDNA3.1-RegIV ΔCRD (LoVo/RegIV ΔCRD group) or empty vector (LoVo/negative group), and then the LoVo/RegIV, LoVo/RegIV ΔCRD, LoVo/negative and LoVo cells were transplanted subcutaneously in nude mice, respectively. The expression levels of RegIV and RegIV/ΔCRD mRNAs in xenograft tissues were detected by RT-PCR. The expression levels of vascular endothelial growth factor (VEGF) and CD34 proteins in xenograft tissues were determined by immunohistochemistry assay. The microvessel density (MVD) in xenograft tissues was calculated. Results: The expression level of VEGF in the xenograft tissues in LoVo/RegIV group was higher than those in the other three groups (P < 0.05), and there was no difference in VEGF expression among the LoVo/RegIV ΔCRD, LoVo and LoVo/negative groups (P > 0.05). The MVD value of xenograft tissues in LoVo/RegIV group was higher than those in the other three groups (P < 0.05), and there was no difference among the LoVo/IV ΔCRD, LoVo and LoVo/negative groups (P > 0.05). Conclusion: RegIV participates the angiogenesis of colorectal carcinoma, and this effect may be correlated with its CRD. Copyright© 2011 by TUMOR.
