The expression and subcellular localization of G protein-coupled receptor 30 in Ishikawa endometrial adenocarcinoma cells
10.3781/j.issn.1000-7431.2011.11.003
- Author:
Jia YE
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- Keywords:
Cell, Ishikawa;
Endometrial neoplasms;
Estrogen receptor;
G protein-coupled receptor 30;
Gene expression
- From:
Tumor
2011;31(11):977-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression and subcellular localization of G protein-coupled receptor 30 (GPR30) in Ishikawa endometrial adenocarcinoma cells. Methods: The expressions of GPR30 mRNA and protein in Ishikawa cells were detected by RT-PCR, Western-blotting and immunofluorescence assay. The positive expression rate of GPR30 in the cell membrane and cytoplasm was determined by flow cytometry (FCM). The subcellular localization of GPR30 labeled with colloidal gold was observed under a transmission electron microscope. Results: The expressions of GPR30 mRNA and protein were both detectable and predominately localized in the cell membrane and cytoplasm of Ishikawa cells. The positive expression rate of GPR30 protein in the cytoplasm [(20.10±0.13)%] was significantly higher than that in the cell membrane [(8.54±0.17)%] (P < 0.01). The subcellular localization of GPR30 protein was mainly in the mesh-like network of cytoplasm, which may refer to rough endoplasmic reticulum. Conclusion: The expression level of GPR30 is higher in cytoplasm than in cell membrane of Ishikawa cells. GPR30 is predominantely localized in the rough endoplasmic reticulum of Ishikawa cells, which suggests an important role of GPR30 in endometrial adenocarcinoma. Copyright© 2011 by TUMOR.
