PLCE1 over-expression inhibits migration of colon cancer SW620 cells and induces their apoptosis
10.3781/j.issn.1000-7431.2011.11.002
- Author:
Xiao-Liang WANG
1
Author Information
1. Department of General Surgery
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Colonic neoplasms;
SW620 cells;
Transfection;
Type C phospholipases
- From:
Tumor
2011;31(11):972-976
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of phospholipase C epsilon-1 (PLCE1 ) over-expression on migration, cell cycle and apoptosis of human colon cancer cells. Methods: The SW620 cells overexpressing PLCE1 were constructed through lipofection. Three groups were designed as follows: parent group (without transfection), control group (transfected with empty plasmid containing green fluorescent protein) and experimental group (transfected with pcDNA-DEST53-PLCE1 plasmid). The expression levels of PLCE1 mRNA and protein in SW620 cells were detected by real-time fluorogenic quantitative-PCR (RFQ-PCR) and Western blotting, respectively. The effect of PLCE 1 over-expression on migation ability of SW620 cells was detected by Transwell chamber assay. The cell cycle distribution and apoptosis rate of SW620 cells were detected by flow cytometry (FCM). The apoptosis was analyzed by using DNA ladder method. Results: The migration ability of SW620 colon cancer cells was inhibited by over-expression of PLCE 1. The numbers of migrated cells in the parent, control and experimental groups were 32.60±2.42, 32.20±3.25 and 8.80±1.72, respectively, and the difference among three groups was significant (P < 0.01). The over-expression of PLCE1 prolonged phase G1 and induced apoptosis. Conclusion: PLCE1 over-expression can inhibit the migration ability of colon cancer cells and induce their apoptosis. PLCE1 over-expression can reduce the malignant degree of colon cancer cells, and this gene may be a new antioncogene related to colon cancer. Copyright© 2011 by TUMOR.
