Over-expression of transducer of ErbB2, 1 inhibits migration and invasion of human lung cancer cell line A549 in vitro
10.3781/j.issn.1000-7431.2012.02.002
- Author:
Ke-Kang SUN
1
Author Information
1. Suzhou Key Laboratory of Radiation Oncology Biology
- Publication Type:Journal Article
- Keywords:
A549;
Cell;
Gene;
In vitro;
Invasion;
Lung neoplasms;
Migration;
TOB 1
- From:
Tumor
2012;32(2):85-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of over-expression of transducer of ErbB2, 1 (TOB1) on migration and invasion of human lung adenocarcinoma cell line A549. Methods: The expression levels of TOB1, phosphatase and tensin homolog deleted on chromosome ten (PTEN), breast cancer metastasis suppressor 1 (BRMS1), ras homolog gene family, member C (RHOC) and non-metastatic cells 1 (NME1) mRNAs in human bronchial epithelial (HBE) cells and ten types of human lung cancer cells were detected by RT-PCR. The recombinant plasmid pcDNA3.0-TOB1 and the empty vector pcDNA3.0 were transfected into A549 cells, respectively. The A549 cells over-expressing TOB1 protein (A549/TOB1 cells) and the A549/PC3 cells (as the control cells) were established. The abilities of migration and invasion of A549/TOB1 cells were determined by wound healing and Transwell assay. The expression levels of PTEN, BRMS1, RHOC and NME1 mRNAs were detected by RT-PCR, and the expression levels of α-N-catenin, β-catenin, γ-catenin, matrix metalloproteinase 2 (MMP2) and MMP9 were detected by Western blotting. Results: The expression levels of TOB1 mRNA in ten types of lung cancer cells were lower than that in HBE cells (P <0.05). The expression level of TOB1 mRNA was positively correlated with the expression levels of PTEN, BRMS1 and NME1 mRNAs (P <0.05). The abilities of migration and invasion of the A549/TOB1 cells were obviously decreased as compared with those of the A549 cells (P <0.05). The expression levels of PTEN, BRMS1 and NME1 mRNAs were higher whereas the expression level of RHOC mRNA was lower in the A549/TOB1 cells as compared with those in the A549 cells (P <0.05). The expression levels of α-N-catenin, β-catenin, γ-catenin, MMP2 and MMP9 proteins in the A549/TOB1 cells were lower than those in the A549 cells (P <0.05). Conclusion: Exogenous TOB1 over-expression can inhibit the abilities of migration and invasion of A549 human lung cancer cells in vitro . This effect may be associated with the downregulation of α-N-catenin, β-cateninm γ-catenin, MMP2 and MMP9 expressions, and which was induced through the regulation of PTEN, BRMS1, RHOC and NME1 expressions by TOB1. Copyright © 2012 by TUMOR.
