Comparative proteomic analysis of human lung adenocarcinoma A549 and A549/DDP cells
10.3781/j.issn.1000-7431.2012.03.004
- Author:
Peng KUANG
1
Author Information
1. Department of Oncology
- Publication Type:Journal Article
- Keywords:
Carcinoma, non-small cell lung;
Cisplatin;
Drug tolerance;
Proteomics
- From:
Tumor
2012;32(3):170-176
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the differentially expressed proteins between A549 cells and DDP (cisplatin)-resistant A549/DDP cells. Methods: A549 cells were exposed to DDP for developing the DDP-resistant A549/DDP cells. The differentially expressed proteins in the A549 and A549/DDP cells were separated and identified by proteomics approach. A part of the differentially expressed proteins were validated by real-time PCR, Western blotting and immunocytochemistry. The functions of these proteins were analyzed by bioinformatics method. Results: There were 8 differentially expressed proteins including POTE, FH (fumarate hydratase), PDE (phosphodiesterase), AKR1C1 (aldo-keto reductase family 1 member C1), DDH2 (dihydrodiol dehydrogenase 2), S100A10, prefoldin subunit 2, and karyopherin beta2- Ran GppNHp nuclear transport complex between A549 and A549/DDP cells, and the difference in expression was more than 5-fold. These proteins were related with cellular metabolism, apoptosis, cell proliferation, detoxification and signal transduction. The validation results of real-time PCR, Western blotting and immunocytochemistry were consistent with the results of proteomic analysis. Conclusion: The differentially expressed proteins between A549 and A549/DDP cells provide some new clues for the study of DDP-resistant lung cancer cells. Copyright© 2012 by TUMOR.
