Effects of over-expression of liver-intestine cadherin on proliferation, invasion and migration of human gastric carcinoma BGC-823 cells
10.3781/j.issn.1000-7431.2012.11.005
- Author:
Hai-Lin XI
1
Author Information
1. Second Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
CDH 17 gene;
Cell adhesion molecules;
Cell proliferation;
Neoplasm invasiveness;
Stomach neoplasms
- From:
Tumor
2012;32(11):880-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of CDH17 (liver-intestine cadherin) over-expression on cell proliferation, invasion and migration of human gastric carcinoma BGC-823 cells in vitro . Methods: The BGC-823 cells over-expressing CDH 17 were constructed using Lipofectamine. The expression levels of CDH 17 mRNA and protein in BGC-823 cells transfected with pcDNA3.1(-)/CDH17 were detected by RFQPCR (real-time fluorogenic quantitative-PCR) and Western blotting, respectively. The proliferative activity of BGC-823 cells was detected by MTT method. The migration and invasive capabilities of BGC-823 cells were detected by scratch-wound migration assay and Transwell invasion assay, respectively. Results: The results of RFQ-PCR and Western blotting revealed that the expression levels of CDH 17 mRNA and protein in BGC-823 cells transfected with pcDNA3.1(-)/CDH17 were over-expressed. The proliferation of BGC-823 cells was increased significantly due to CDH17 over-expression (P < 0.05). The invasive and migration capabilities of BGC-823 cells transfected with pcDNA3.1(-)/CDH17 were also significantly strengthened (P < 0.05). Conclusion: Overexpression of CDH 17 may play an important role in the proliferation, invasion and migration of human gastric carcinoma BGC-823 cells and it can promote the oncogenesis and progression of human gastric carcinoma. Copyright © 2012 by TUMOR.
