Inhibitory effect of miR-143 on migration of pancreatic cancer PANC-1 cells in vitro and its mechanism
10.3781/j.issn.1000-7431.2012.11.001
- Author:
Wen-Jie CHENG
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell migration assays;
Cell proliferation;
MicroRNAs;
Mitogen-activated protein kinases;
Pancreatic neoplasms;
TAK1
- From:
Tumor
2012;32(11):855-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of miR-143 (microRNA-143) on biological activity of pancreatic cancer PANC-1 cells. Methods: MiR-143 mimics were chemically synthesized and transfected transiently into PANC-1 cells by LipofectAMINE™ 2000. The expression of miR-143 in pancreatic cancer cells was examined by real-time fluorescence quantitative PCR. The cell proliferation was measured by MTT method. The apoptosis was detected by flow cytometry. Transwell and wound healing assays were performed to examine the migration ability of PANC-1 cells. The dual luciferase reporter vectors containing 3'-UTR (3'-untranslated region) with miR-143 binding site of wild type or mutant TAK 1 (transforming growth factor-β activated kinase 1) were constructed by using Dual-Luciferase® Reporter Assay System, and then the relative activity of Renilla reniformis luciferase was detected to confirm the binding site of miR-143 on TAK1's mRNA. Results: The expression of miR-143 in miR-143 mimicstransfected PANC-1 cells was significantly up-regulated. The transfection of miR-143 failed to influence the activities of proliferation and apoptosis of PANC-1 cells (P > 0.05), and however, the migration ability of PANC-1 cells was reduced (P < 0.05). Compared with negative control or wild type TAK 1 or mutant TAK 1, cotransfection with miR-143 and wild type TAK 1 3'-UTR could significantly decrease the relative activity of Renilla reniformis luciferase. Conclusion: miR-143 can restrain the migration ability of PANC-1 cells in vitro , in which TAK 1 may be one of the target genes. Copyright © 2012 by TUMOR.
