Newcastle disease virus promotes expression of TNF-related apoptosis-inducing ligand in human NK cells
10.3781/j.issn.1000-7431.2012.12.005
- Author:
Jun YIN
1
Author Information
1. Department of Microbiology
- Publication Type:Journal Article
- Keywords:
Experimental;
Immunotherapy;
Liver neoplasms;
Natural killer cells;
Newcastle disease virus;
TNF-related apoptosis-inducing ligand
- From:
Tumor
2012;32(12):974-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The purpose of this study is to investigate the mechanism for the TRAIL (TNFrelated apoptosis-inducing ligand) expression in NK (natural killer) cells stimulated by NDV (Newcastle disease virus). Methods: Pure NK cells were isolated by using MACS (magnetic activated cell sorting). The cytotoxicity of NK cells stimulated by NDV was detected by LDH releasing assay. TRAIL transcription levels in NK cells stimulated by NDV were detected through RFQ-PCR (real-time fluorogenic quantitative-PCR). TRAIL expression on NK cell membrane stimulated with NDV for 16 h was analyzed by FCM (flow cytometry). The level of IFN (interferon) -γ was determined by ELISA after NK cells were stimulated by NDV. TRAIL expression on NK cell membrane was analyzed by FCM after applying the anti-human IFN-γ while NDV was added into NK cell medium. Results: The purity of NK cells detected by FCM was (90.60± 1.15)%. NDV can enhance the cytotoxicity of NK cells after stimulation for 16 h, and the cytotoxicity of NK cells reached (22.28±0.84)% after stimulation with 204.8HU NDV. TRAIL mRNA expression levels were increased after stimulation with various concentrations of NDV for 4 h. TRAIL expression on NK cell membrane was significantly increased after stimulation with NDV for 16 h. IFN-γ level was significantly increased after NK cells were stimulated by NDV, consistent with the concentration of NDV and it was time-dependent. IFN-γ level reached a peak of 796.47±37.87 pg/mL after stimulation with 25.6 HU NDV for 16 h. TRAIL expression was significantly decreased after IFN-γ was neutralized. Conclusion: NDV can enhance the expression of IFN-γ in NK cells, and IFN-γ can up-regulate the TRAIL expression. This is one of the mechanisms for TRAIL expression on NK cells stimulated by NDV. Besides this mechanism, there exists another mechanism for TRAIL expression on NK cells directly stimulated by NDV. Copyright © 2012 by TUMOR.
