Detection of Th17 cells in peripheral blood of patients with multiple myeloma and its clinical significance
10.3781/j.issn.1000-7431.2013.05.013
- Author:
Yuan SONG
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- Keywords:
Helper T cell 17;
Interleukins;
Multiple myeloma;
T-lymphocytes
- From:
Tumor
2013;33(5):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine the Th17 cells (helper T cells 17) in peripheral blood of patients with MM (multiple myeloma) and explore its clinical significance. Methods: The peripheral blood samples from 39 patients with MM and 30 healthy volunteers were collected. The proportion of Th17 cells in CD4 + T cells was determined by flow cytometry. The expression of ROR-γt (retinoid-related orphan receptor-γt) mRNA, a specific transcription factor of Th17 cells, was detected by RT-PCR. The plasma concentration of IL-17 (interleukin-17) was measured by ELISA assay. Results: The proportion of Th17 cells, the expression level of ROR-γt mRNA and the concentration of IL-17 in peripheral blood in patients with MM were higher than those in the healthy volunteers [(3.509±0.594)% vs (2.913±0.846)%, 0.568±0.177 vs 0.468±0.163, 27.200±4.666 vs 23.758±7.410 pg/mL; all P values < 0.05). The proportion of Th17 cells was not correlated with the gender, age, disease type, globulin, immune globulin, light chain, M-protein and the proportion of plasmocytes of the patients with MM (P > 0.05), but correlated with ISS (international staging system) stage, β2-microglobulin level and the therapeutic response (P < 0.05). Conclusion: Th17 cells may be involved in the occurrence and development of MM. Detection of proportion of Th17 cells may contribute to the ajudgement of disease severity, therapeutic response and the prognosis of patients with MM. Copyright © 2013 by TUMOR.
