Docetaxel inhibits the migration and invasion of breast cancer cells by suppressing filopodia formation
10.3781/j.issn.1000-7431.2013.09.005
- Author:
Li-Quan OUYANG
1
Author Information
1. Department of Thyroid and Breast Surgery
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Cell movement;
Docetaxel;
MDA-MB-231 cells;
Pseudopodia
- From:
Tumor
2013;33(9):776-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of docetaxel and ADM (doxorubicin) on migration, invasion and filopodia formation of breast cancer cells. Methods: Triple-negative breast cancer MDA-MB-231 cells were divided into three groups: blank control group, docetaxel group and ADM group. CCK-8 (cell counting kit-8) assay was used to determine the IC10 (inhibitory concentration of 10%) values of docetaxel and ADM. Abilities of invasion and migration were detected by Transwell assay under IC10 of docetaxel and ADM. The effect of these concentrations on cellular proliferation was assayed by flow cytometry to detect the cell cycle. The rhodamine-phalloidin was used to stain cell cytoskeleton to observe filopodia formation after treatment with these two drugs. Results: The IC10 values of docetaxel and ADM in MDA-MB-231 cells were 1.0 ng/mL and 0.5 μg/mL, respectively. These two concentrations had no effects on cellular proliferation and cell cycle (P > 0.05). Docetaxel (1.0 ng/mL) could inhibit migration and invasion of tumor cells more effectively than ADM (0.5 μg/mL). Docetaxel inhibited filopodia formation of triple-negative breast cancer cells more effectively with a statistical significance. Conclusion: Docetaxel can obviously inhibit the migration and invasion of breast cancer cells as compared with ADM, which may be associated with suppression of filopodia formation by these agents. Copyright © 2013 by TUMOR.
