Effect of Scutellaria barbata D. Don on expression of ADAM10 in primary liver cancer tissues in rats
10.3781/j.issn.1000-7431.2013.10.006
- Author:
Zhi-Qiang KANG
1
Author Information
1. Department of Oncology
- Publication Type:Journal Article
- Keywords:
A disintegrin and metalloproteinase 10;
Liver neoplasms;
Matrine;
Rats, Wistar;
Scutellarta barbata
- From:
Tumor
2013;33(10):879-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Scutellaria barbata D. Don on the expression of ADAM10 (a disintegrin and metalloproteinase 10) in primary liver cancer tissues in rats. Methods: Total of 120 Wistar rats were randomly divided into four groups: normal, model, matrine and Scutellaria barbata D. Don groups. The model of rat bearing primary liver cancer was induced by diethylnitrosamine. The rats in the matrine group and Scutellaria barbata D. Don group were fed with matrine and Scutellaria barbata D. Don powder, respectively. The rats in the normal group and model group were fed with 0.9% sodium chloride solution. The number of hepatocellular carcinoma nodules was calculated, and the tumor growth inhibition rate was also calculated. The pathologic changes of hepatic tissues in rats of each group were observed by hematoxylin and eosin staining. The expressions of ADAM10 in liver cancer tissues in these four groups were determined by immunohistochemistry and Western boltting. Results: The number of hepatocellular carcinoma nodules of the matrine group and Scutellaria barbata D. Don group was less than that of the model group (P < 0.05) with the appearance of apoptotic bodies. The tumor growth inhibition rate of Scutellaria barbata D. Don group was higher than that of the matrine group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of the model group were significantly higher than those of the normal group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of matrine group and Scutellaria barbata D. Don group were lower than those of the model group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of Scutellaria barbata D. Don group were lower than those of matrine group (P < 0.05). Conclusion: Scutellaria barbata D. Don may inhibit the growth of primary liver cancer in rats through down-regulating the expression of ADAM10. Copyright © 2013 by TUMOR.
