Effects of diallyl trisulfide on cell cycle and apoptosis of human osteosarcoma cell line Saos-2
10.3781/j.issn.1000-7431.2013.03.002
- Author:
Yong-Kui ZHANG
1
Author Information
1. Department of Orthopedics
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell cycle;
Diallyl trisulfide;
Garlic;
Osteosarcoma;
Proteomics
- From:
Tumor
2013;33(3):214-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of DATS (diallyl trisulfide) on cell proliferation, cell cycle and apoptosis of human osteosarcoma cell line Saos-2, and to explore their potential molecular mechanisms. Methods: Saos-2 cells were treated with 25, 50 and 100 μmol/L DATS for 24, 48, 72, and 96 h. The change of cell morphology was observed under an inverted microscope. The effect of DATS on the proliferation of Saos-2 cells was detected by MTT assay. After treatment with DATS at desired concentrations for various time intervals, the cell cycle and apoptotic rate of Saos-2 cells were analyzed by flow cytometry. The expressions of DATS-sensitive proteins in Saos-2 cells were systematically detected using 2-DE (two-dimensional gel electrophoresis) and mass spectrometry analysis. Results: DATS could inhibit the proliferation of Saos-2 cells in a dose- and time-dependent manner. Moreover, DATS could arrest the cell cycle at phase G0/G1 and induce the apoptosis in a time- and concentrationdependent manner. A total of 27 unique DATS-sensitive proteins, including 18 downregulated proteins and 9 upregulated proteins, were found in Saos-2 cells. Conclusion: DATS can suppress the proliferation of Saos-2 cells by blocking cell cycle progression and inducing apoptosis. The results of proteomics not only suggest the anticarcinogenic mechanism of DATS, but also provide a novel insight into the therapeutic targets for gene therapy of osteosarcoma. Copyright © 2013 by TUMOR.
