The value of combined detection of p16INK4A, Brn3a, telomerase and other biomarkers in cervical cytology for predicting the progression of cervical neoplasia
10.3781/j.issn.1000-7431.2013.12.011
- Author:
Chu-Yue ZHANG
1
Author Information
1. Department of Obstetrics and Gynecology
- Publication Type:Journal Article
- Keywords:
Cervical intraepithelial neoplasia;
Diagnosis, differential;
Genes;
Oncogenes;
P16INK4A;
Telomerase;
Transcription factor Brn-3A
- From:
Tumor
2013;33(12):1101-1107
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expressions of p16INK4A, Brn3a, telomerase and human cervical cancer oncogene (HCCR) in cervical intraepithelial neoplasia (CIN), and to estimate their values for predicting the progression of cervical lesions. Methods: Paraffin specimens of 262 cases were all from the residue of patients' liquid-based cytology test (LCT) samples, including 30 normal cases, 80 cases of atypical squamous cells of undetermined significance (ASCUS), 72 cases of low-grade squamous intraepithelial lesions (LSIL), 50 cases of high-grade squamous epithelial lesions (HSIL) and 30 cases of invasive squamous cell carcinoma. The expression levels of p16INK4A, Brn3a, telomerase and HCCR in these samples were detected using immunocytochemistry. Results: The expression levels of p16INK4A and Brn3a in ASCUS and LSIL specimens were significantly higher than those in normal cells (P < 0.05). Compared to ASCUS and LSIL, the expression levels of p16INK4A, Brn3a, telomerase and HCCR in HSIL and invasive squamous cell carcinoma were significantly higher (P < 0.05). Among ASCUS, both the expression of p16 INK4A in cases that had pathological diagnosis of CIN1 and the expressions of p16INK4A and Brn3a in cases that had pathological diagnosis of CIN2+ were significantly higher than those had pathological diagnosis of chronic cervicitis or cervical condyloma (P < 0.05). Among LSIL, the expressions of p16INK4A and Brn3a in cases that had pathological diagnosis of CIN2+ were significantly higher than those had pathological diagnosis of chronic cervicitis or cervical condyloma (P < 0.05). Moreover, the expressions of p16INK4A and Brn3a in ASCUS and LSIL had a strong relationship (r = 0.580, P < 0.001). Conclusion: The expression levels of p16INK4A, Brn3a, telomerase and HCCR can help distinguish HSIL and invasive squamous cell carcinoma from ASCUS and LSIL, and combined detection of p16INK4A and Brn3a maybe assist in predicting the progression of ASCUS and LSIL. Copyright © 2013 by TUMOR.
