The correlation between the expressions of hypoxia-inducible factor-1α and centromere protein W in glioma tissues and cells
10.3781/j.issn.1000-7431.2014.33.437
- Author:
Chang-Chun LIAO
1
Author Information
1. Department of Neurosurgery, Second Affiliated Hospital, Nanchang University
- Publication Type:Journal Article
- Keywords:
Centromere protein-W;
Glioma;
Hypoxia-inducible factor 1, alpha subunit;
RNA, small interfering
- From:
Tumor
2014;34(11):1045-1051
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the correlation between the expressions of hypoxia-inducible factor-1α (HIF-1α) and centromere protein-W (CENP-W) in glioma tissues and cells. Methods: The expressions of HIF-1α and CENP-W in high-grade glioma tissues, low-grade glioma tissues and adjacent normal brain tissues were detected by immunohistochemistry, then the relationships of these two proteins' expressions with the clinicopathologic features of patients with glioma were investigated, and the correlation between the expressions of HIF-1α and CENP-W was analyzed. After using cobalt dichloride to inhibit the degradation of HIF-1a protein in glioma U251cells, the expressions of CENP-W mRNA and protein were detected by realtime fluorescene-based quantitative PCR (RFQ-PCR) and Western blotting, respectively. After transfection with the specific small interference RNA (siRNA) targeting HIF-1α gene to down-regulate the expression of HIF-1a in U251cells, the changes of CENP-W mRNA and protein levels were detected by RFQ-PCR and Western bloting, respectively. After transfection with the specific siRNA targeting CENP-W gene to down-regulate the expression of CENP-W in U251cells, the changes of HIF-1a mRNA and protein levels were detected by RFQ-PCR and Western bloting, respectively. Results: The expression levels of HIF-1a and CENP-W in glioma tissues were significantly higher than those in normal brain tissues (P < 0.05). The expression levels of these two proteins were positively correlated with the pathological grade of glioma (P < 0.05). There was a positive correlation between the expression levels of HIF-1a and CENP-W (r = 0.709, P < 0.05). After the inhibition of HIF-1α protein degradation by cobalt dichloride, the mRNA and protein expressions of HIF-1a and CENP-W were significantly up-regulated in glioma U251cells (P < 0.01). The expressions of CENP-W mRNA and protein were down-regulated when the expression of HIF-1a in U251cells was suppressed by siRNA targeting HIF-1α gene (P < 0.01). But the expression levels of HIF-1a mRNA and protein had no changes in U251cells when the expression of CENP-W was suppressed by siRNA targeting CENP-W gene (P >0.05). Conclusion: Both of HIF-1α and CENP-W expressions are positively correlated with the pathological grade of glioma. HIF-1α can regulate the expression of CENP-W.
