Effects of 5-Aza-dC on KISS1 gene expression and the abilities of invasion and migration of human colorectal cancer cell line HCT116
10.3781/j.issn.1000-7431.2014.01.005
- Author:
Shao-Qin CHEN
1
Author Information
1. Department of Gastrointestinal Surgery, Fujian Medical University
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Deoxycytidine;
KISS1 gene;
Methylation;
Neoplasm metastasis
- From:
Tumor
2014;34(1):26-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of methylation of KISS1 gene promoter and KISS1 expression on biological characteristics of colorectal cancer HCT116 cells induced by methyltransferase inhibitor 5-aza-2′-deoxycytidine (5-Aza-dC). Methods: The promoter methylation status of KISS1 gene and its mRNA and protein expressions in HCT116 cells were detected by methylation-specific PCR, real-time fluorogenic quantitative-PCR and Western blotting, respectively; after treatment with 5-Aza-dC in vitro. The abilities of invasion and migration of HCT116 cells were detected by Transwell assay, and the cell proliferation was detected by Cell Counting Kit-8 (CCK-8). Results: The demethylation of KISS1 promoter was induced after treatment with 5-Aza-dC. The expressions of KISS1 mRNA and protein in HCT116 cells were significantly increased in a dose-dependent manner after treatment with different doses of 5-Aza-dC for 5 days as compared with those without treatment with 5-Aza-dC (P < 0.05). The differences in the expressions of KISS1 mRNA and protein were not statistically significant between 5 μmol/L of 5-Aza-dC intervention group and 10 μmol/L of 5-Aza-dC intervention group (P > 0.05). The abilities of invasion and migration of HCT116 cells after 5-Aza-dC treatment were also reduced (P < 0.05). Conclusion: The DNA methyltransferase inhibitor 5-Aza-dC can reverse the methylation of KISS1 promoter, and reduce the abilities of invasion and migration of HCT116 cells by up-regulating the expression of KISS1. Copyright© 2014 by TUMOR.
