Effects of miR-192 on the expression of ZEB2 and the abilities of migration and invasion of colorectal cancer cells
10.3781/j.issn.1000-7431.2014.01.003
- Author:
Wei-Yun WU
1
Author Information
1. Department of Gastroenterology, Guangdong Medical College
- Publication Type:Journal Article
- Keywords:
Cells, SW480, SW620;
Colorectal neoplasms;
MicroRNAs;
Neoplasm invasiveness;
Zinc-finger E-box binding homeobox 2
- From:
Tumor
2014;34(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of miR-192 on the expression of zinc-finger E-box binding homeobox 2 (ZEB2) and the abilities of migration and invasion of colorectal cancer (CRC) SW480 and SW620 cells. Methods: After transfection of miR-192 mimics into SW480 and SW620 cells, the expression levels of miR-192 and ZEB2 mRNA and ZEB2 protein were detected by real-time fluorescence quantitative RT-PCR and Western blotting, respectively. The abilities of migration and invasion of SW480 and SW620 cells after transfection with miR-192 mimics were determined by wound healing and Transwell assays, respectively. The recombinant vector pmiR-ZEB2-wt and miR-192 mimics were co-transfected into SW480 cells. The binding site of 3′-untranslated region (3′-UTR) of ZEB2 gene with miR-192 was verified by Dual Luciferase™ Reporter Gene Assay. Results: As compared with the negative control (NC) group (transfection with miR-192 mimics-NC), the expression levels of miR-192 in SW480 and SW620 cells after transfection with miR-192 mimics were increased (P < 0.05), and the expression levels of ZEB2 mRNA and protein were decreased (P < 0.05), as well as the abilities of migration and invasion of SW480 and SW620 cells were inhibited (P < 0.05). The Dual Luciferase™ Reporter Gene Assay revealed that the luciferase activity in SW480 cells after co-transfection with recombinant vector pmiR-ZEB2-wt and miR-192 mimics was inhibited (P < 0.05), which indicated that the 3′-UTR of ZEB2 harbored a binding site for miR-192. Conclusion: ZEB2 may be one of the target genes for miR-192. Overexpression of miR-192 may down-regulate the ZEB2 expression and inhibit the migration and invasion of SW480 and SW620 cells. Copyright© 2014 by TUMOR.
