The effects of lung cancer conditioned medium on the proliferation of UCB-MSCs and the expression of phosphorylated p38MAPK
10.3781/j.issn.1000-7431.2014.02.004
- Author:
Zhe YUAN
1
Author Information
1. Department of Blood Transfusion
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Fetal blood;
Lung neoplasms;
Mesenchymal stem cells;
P38 Mitogen activated protein kinases
- From:
Tumor
2014;34(2):121-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of lung cancer A549 cells conditioned medium on the proliferative characteristics of umbilical cord blood-mesenchymal stem cells (UCB-MSCs) and the expression of phospho-p38 mitogen activated protein kinase (p-p38MAPK) in UCB-MSCs. Methods: Primary culture system of UCB-MSCs from human umbilical cord blood was established. UCB-MSCs were divided into control (incubated with UCB-MSCs culture medium), low concentration (incubated with 50% A549 cells conditioned medium and 50% UCB-MSCs culture medium) and high concentration (incubated with 100% A549 cells conditioned medium) groups. The abilities of proliferation and migration of UCB-MSCs in each group were detected by MTT method and wound healing assay, respectively. The cell cycle distribution and the expression level of p-p38MAPK protein of UCB-MSCs in each group were analyzed by flow cytometry and Western blotting, respectively. Results: The UCB-MSCs were successfully isolated and cultured. As compared with the control group, the abilities of proliferation and migration of UCB-MSCs in the low concentration and the high concentration groups were increased (P < 0.05). The percentage of the cells in G0/G 1 phase in the high concentration group was lower than that in the control group, while the percentage of the cells in G2/M phase was increased (all P < 0.05). The percentages of the cells in S phase in the low concentration and the high concentration groups were higher than that in the control group (P < 0.05). The expression levels of p-p38MAPK protein of UCB-MSCs in the low concentration and the high concentration groups were up-regulated (P < 0.05). Conclusion: Lung cancer A549 cells conditioned medium can stimulate the proliferation and the migration of UCB-MSCs and up-regulate the expression level of p-p38MAPK protein. Copyright © 2014 by TUMOR.
