The expression of thrombospondin 1 and its gene hypermethylation in laryngeal squamous cell cancer
10.3781/j.issn.1000-7431.2014.03.011
- Author:
Chuang HUANG
1
Author Information
1. Department of Head and Neck Surgery, Chongqing Cancer Institute
- Publication Type:Journal Article
- Keywords:
DNA methylation;
Gene expression;
Laryngeal neoplasms;
Lymph node involvement;
Neoplasm staging;
Thrombospondin 1
- From:
Tumor
2014;34(3):264-270
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mRNA and protein expressions of thrombospondin 1 (THBS1) gene and the DNA methylation of its promoter in laryngeal squamous cell cancer (LSCC), and to analyze their relationship to clinicopathological features of LSCC. Methods: Sixty-six pairs of fresh LSCC tissues and the matching adjacent normal tissues were taken from 66 cases of LSCC. The mRNA and protein expressions of THBS1 gene were detected by revere transcription PCR and Western blotting assay, respectively. Then their relationship to clinicopathological features of LSCC was analyzed by one-way ANOVA and t test. The methylation of THBS1 gene promoter was detected by the DNA methylation specific PCR, and its relationship to clinicopathological features of LSCC was analyzed by ?2 test. Results: The mRNA and protein expressions of THBS1 gene in LSCC tissues were significantly decreased as compared with those in the adjacent normal tissues (P < 0.05), and these expressions were also associated with lymph node involvement and TNM staging (P < 0.05). The positive rate of the hypermethylation of THBS1 gene in the LSCC tissues (48.50%±6.15%) was significantly higher than that in the matching adjacent normal tissues (6.06%±2.92%) (P < 0.05). The mRNA and protein expressions of THBS1 gene were associated with the hypermethylation of its gene promoter (P < 0.05). THBS1 gene hypermethylation status in LSCC tissues was associated with lymph node involvement and TNM staging (P < 0.05). Conclusion: The decreased expressions of THBS1 mRNA and protein and the high methylation of its gene promoter may play crucial roles in carcinogenesis and development of LSCC. Copyright © 2014 by TUMOR.
