Aberrant expression of long intergenic non-coding RNAs in human colorectal cancer
10.3781/j.issn.1000-7431.2014.03.008
- Author:
Zhi-Qiang GU
1
Author Information
1. Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Long non-coding RNAs;
Microchip analytical procedures;
System biology
- From:
Tumor
2014;34(3):245-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression profile of long intergenic non-coding RNAs (lincRNAs) in human colorectal cancer (CRC). Methods: The expressions of long non-coding RNAs (lncRNAs) and mRNAs in four CRC tissues and para-cancerous normal colorectal mucosa tissues were detected by microarray analysis. Clustering heatmaps were made to profile the lincRNAs and mRNAs expressions by MeV 4.6 software. The signal pathways were analyzed by SBC Analysis System (SAS). The linc-UCC, linc-DCC and MIR143HG expression levels were detected by real-time fluorogenic quantitative-PCR (RFQ-PCR). Results: One hundred twenty-four lincRNAs and 1 583 mRNAs out of 42 545 RNAs detected by microarray assay were identified as differentially expressed between CRC tissues and para-cancerous normal colorectal mucosa tissues. Pathway analysis indicated that 29 potential pathways corresponded to these aberrant lincRNAs. linc-UCC was frequently up-regulated in CRC tissues and its high expression had a significant correlation with lymph node metastasis. linc-DCC and MIR143HG were down-regulated in CRC tissues and the positive correlation was shown between them. Conclusion: These results suggest that the differences in lincRNAs expression profiles may provide a new target for diagnosis and treatment of CRC. Copyright © 2014 by TUMOR.
