The effect of RNA binding protein Lsm4 on the proliferation and migration of esophageal carcinoma cell line EC109
10.3781/j.issn.1000-7431.2014.03.002
- Author:
Yin LONG
1
Author Information
1. Department of Clinical Laboratory, Tenth People's Hospital of Tongji University
- Publication Type:Journal Article
- Keywords:
Cell movement;
Cell proliferation;
Esophageal neoplasms;
RNA-binding proteins
- From:
Tumor
2014;34(3):204-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Lsm4 gene interference on the proliferation and migration of esophageal carcinoma EC109 cells, and to explore its possible mechanism. Methods: The expression of Lsm4 mRNA in esophageal cancer tissues and para-carcinoma tissues was detected by real-time fluorescence quantitative-PCR (RFQ-PCR). After transfection with Lsm4-small interference RNA (siRNA) targeting human Lsm4 gene or a negative control (NC) (NC-siRNA), the expression level of Lsm4 mRNA in EC109 cells was detected by RFQ-PCR, and the abilities of cell proliferation and colony formation were determined by MTT and colony formation assay, respectively. The ability of migration of EC109 cells after transfection with Lsm4-siRNA was examined by Transwell assay, and the expression levels of Lsm4, proliferating cell nuclear antigen (PCNA) and vimentin proteins were detected by Western blotting. Results: The expression level of Lsm4 mRNA in esophageal cancer tissues was higher than that in para-carcinoma tissues (P < 0.05). The expression levels of Lsm4 mRNA and protein of EC109 cells in Lsm4-siRNA transfection group were lower than those in NC-siRNA group (P < 0.01). The abilities of proliferation and colony formation of EC109 cells in Lsm4-siRNA transfection group were inhibited (P < 0.05), while the ability of migration was improved (P < 0.01). As compared with NC group, the expression level of PCNA was down-regulated (P < 0.05), and the expression level of vimentin was up-regulated (P < 0.01). Conclusion: The expression of Lsm4 mRNA is higher in esophageal carcinoma tissues. Lsm4 may regulate the growth and migration of EC109 cells. Copyright © 2014 by TUMOR.
