Early diagnostic value of plasma miR-155, miR-196a, miR-21 and miR-210 in patients with pancreatic cancer
10.3781/j.issn.1000-7431.2015.33.333
- Author:
Yingxia HU
1
Author Information
1. Yunnan Institute of Digestive Diseases, Department of Gastroenterology, First Affiliated Hospital, Kunming Medical University
- Publication Type:Journal Article
- Keywords:
Early diagnosis;
MicroRNAs;
miR-155;
miR-196a;
miR-21;
miR-210;
Pancreatic neoplasms;
Plasma
- From:
Tumor
2015;35(10):1135-1143
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the clinical value of the plasma microRNA-155 (miR-155), miR-196a, miR-21 and miR-210 in early diagnosis of patients with pancreatic cancer. Methods: The real-time fluorescent quantitative-PCR was performed to detect the levels of miR-155, miR-196a, miR-21 and miR-210 in plasma samples from sixty patients with pancreatic cancer, twenty patients with chronic pancreatitis, and ten healthy volunteers, as well as the levels of four miRNAs in cancer tissue specimens from ten patients with pancreatic cancer. The serum tumor markers carbohydrate antigen 199 (CA199), CA242 and carcino-embryonic antigen (CEA) were simultaneously detected. The relative expression levels of four miRNAs in plasma among these three groups were compared, and the relationship between miRNA levels in plasma and their levels in pancreatic cancer tissues were statistically analyzed. Finally, the diagnostic efficiency of plasma miR-155, miR-196a, miR-21 and miR-210 for pancreatic cancer was evaluated. Results: The relative expression levels of plasma miR-155, miR-196a, miR-21 and miR-210 in pancreatic cancer group were significantly higher than those in the chronic pancreatitis group and the healthy control group (all P < 0.01). The relative levels of miR-155, miR-196a, miR-21 and miR-210 in pancreatic cancer tissues were higher than those in plasma from the same patients with pancreatic cancer, but there were no statistically significant differences (all P > 0.05). The area under receiver operating characteristic (AUC-ROC) curve of plasma miR-155, miR-196a, miR-21 and miR-210 in diagnosis of pancreatic cancer indicated that these four miRNAs had diagnostic value independently (all P < 0.01), in which miR-155 had the highest diagnostic efficiency. The binary Logistic regression model showed that combined detection of plasma miR-196a and miR-210 was more effective in diagnosis of stageI pancreatic cancer as compared with CA199. Conclusion: The plasma miR-155, miR-196a, miR-21 and miR-210 levels are effective to distinguish pancreatic cancer from non-pancreatic cancer. The combined detection of miR-196a and miR-210 may become a promising method for early diagnosis of pancreatic cancer.
