Inhibiting effect of menin protein on the growth of human gastric cancer cell line AGS through PI3K/Akt signal transduction pathway
10.3781/j.issn.1000-7431.2015.11.077
- Author:
Feng REN
1
Author Information
1. Department of Clinical Laboratory, Fourth People's Hospital of Wuxi, Affiliated Hospital of Jiangnan University
- Publication Type:Journal Article
- Keywords:
Adenovirus infection;
Multiple endocrine neoplasia type 1;
Signal transduction;
Stomach neoplasms
- From:
Tumor
2015;35(4):390-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of menin protein encoded by the multiple endocrine neoplasia type I (MEN 1) gene on proliferation of human gastric cancer AGS cells and its possible machanism. Methods: An adenovirus encoding MEN 1 gene was used to infect human gastric cancer AGS cells. The expression level of menin protein in AGS cells infected with recombinant adenovirus Ad-MEN1 was detected by Western blotting and immunofluorescence. The proliferation of AGS cells was tested by MTT method. The cell cycle distribution and apoptosis of AGS cells were analyzed by flow cytometry (FCM). The expressions of phospho-Akt (p-Akt) and nuclear factor-kappa B (NF-κB) which were related to phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB) signal pathway were detected by Western blotting. Results: The results of Western blotting and immunofluorescence showed that the expression of menin protein in gastric cancer cell line AGS was increased after Ad-MEN1 infection (P < 0.05). The infective efficiency of recombinant adenovirus Ad-MEN1 was the highest when the multiplicity of infection was 60 in AGS cells, and the cell proliferation was obviously inhibited (P < 0.05). The percentage of cells was increased in phase G0/G1 and decreased in phase S (P < 0.05). However, the apoptosis rate of AGS cells after infection with Ad-MEN1 was not significantly changed (P > 0.05). The expressions of p-Akt and NF-κB p65 in AGS cells were decreased after the expression of menin in AGS cells was increased (P < 0.05). Conclusion: This study reveals that over-expression of menin can inhibit the growth of human gastric cancer cell line AGS. This machanism may be related to the inhibition of PI3K/Akt and NF-κB signal transduction pathways.
