Inhibition effect of targeting fusion protein TAT-OSBP-MKK6(E) on human ovarian xenograft tumor in nude mice
10.3781/j.issn.1000-7431.2015.11.658
- Author:
Jin YUAN
1
Author Information
1. Department of Gynecology and Obstetrics, Guangzhou First People's Hospital
- Publication Type:Journal Article
- Keywords:
Animal experimentation;
Apoptosis;
Mice, nude;
Ovarian neoplasms;
Recombinant fusion proteins
- From:
Tumor
2015;35(2):161-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibition effect of trans-activator of transcription (TAT)-ovarian cancer specific binding peptide (OSBP)-mitogen-activated protein kinase kinase 6 mutant (E) [MKK6(E)] on human ovarian xenograft tumor in nude mice and its possible mechanism. Methods: The subcutaneous xenograft tumor model of human ovarian cancer HO8910 cells in nude mice was established. Then the tumor-bearing nude mice were randomly divided into three groups: treatment group [TAT-OSBP-MKK6(E) was administered intraperitoneally], negative control group (TAT-OSBP was administered intraperitoneally) and the blank control group (the saline was administered intraperitoneally). The tumor growth, tumor volume and body weight of nude mice of three groups were observed. The apoptosis and the expressions of proliferating cell nuclear antigen (PCNA) and p38 of xenograft tumors were examined by TUNEL method, immunohistochemistry and Western blotting, respectively. Results: As compared with the negative control and the blank control groups, the growth rate of xenograft tumor in the treatment group was decreased significantly (P <0.05). The volume of xenograft tumor and the body weight of nude mice in the treatment group were decreased significantly as compared with those in the negative control and the blank control groups (both P <5); there was no difference between the negative control and the blank control groups (P >5). The apoptosis index (AI) value in the treatment group was higher than those in the negative control and the blank control groups (P <5). The positive rate of PCNA expression in the treatment group was lower than those in the negative control and the blank control groups, and the expression level of p38 protein was opposite (all P < 0.05). The AI value, the positive rate of PCNA expression and the expression level of p38 protein were not significantly different between the negative control and the blank control groups (all P > 0.05). Conclusion: TAT-OSBP-MKK6(E) can inhibit the growth and the expression of PCNA in subcutaneous xenograft tumor of ovarian cancer in nude mice. This effect may be related to promoting apoptosis.
