The expression of miR-98 in glioma and the promotion effect on invasion of glioma cells by miR-98 down-regulation
10.3781/j.issn.1000-7431.2016.33.496
- Author:
Yanghua FAN
1
Author Information
1. Department of Neurosurgery, Second Affiliated Hospital of Nanchang University
- Publication Type:Journal Article
- Keywords:
Cell migration assays;
Glioma;
MicroRNAs;
Neoplasm invasiveness
- From:
Tumor
2016;36(10):1156-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the expression of miR-98 in glioma tissues and investigate the effect of miR-98 on the invasion and migration of human glioma cell line U251. Methods: The expressions of miR-98 in glioma tissues and normal brain tissues were detected by real-time fluorescent quantitative PCR. After co-transfection with miR-98 inhibitor and wide type inhibitor of nuclear factor kappa B kinase epsilon (IKBKE) 3'-untranslated region (UTR) or mutation type IKBKE 3'-UTR recombination vector, the specific binding ability of miR-98 to 3'-UTR in IKBKE gene was examined by luciferase gene reporter system. The expression levels of miR-98, IKBKE mRNA and IKBKE protein in glioma cell line U251 after transfection with miR-98 inhibitor were measured by real-time fluorescent quantitative PCR and Western blotting, respectively.The abilities of migration and invasion of U251 cells after transfected with miR-98 inhibitor were detected by Transwell assay. The expression of miR-98 in U251 cells after transfection with IKBKE siRNA was detected by real-time fluorescent quantitative PCR. Results: The expression of miR-98 in glioma tissues was lower than that in normal brain tissues (P < 0.05). The fluorescence intensity of U251 cells co-transfected with miR-98 inhibitor and wide type IKBKE recombination vector was improved (P < 0.05).The expression of miR-98 was down-regulated, and the expressions of IKBKE mRNA and protein were upregulated in U251 cells after transfection with miR-98 inhibitor (all P<0.05). While the abilities of migration and invasion of U251 cells after transfection with miR-98 inhibitor were increased (both P<0.05). There was no change of expression of miR-98 in U251 cells after inhibition of IKBKE expression (P > 0.05). Conclusion: The expression of miR-98 is low in glioma tissues. miR-98 inhibitor may promote the invasion and migration of glioma U251 cells by regulation of IKBKE expression.
