Ubiquitin ligase TRIM46 promotes the proliferation and invasion of colorectal cancer cells
10.3781/j.issn.1000-7431.2017.11.785
- Author:
Qian LIANG
1
Author Information
1. Department of Gastroenterology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Digestive Disease
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Colorectal neoplasms;
Neoplasm invasiveness;
TRIM46
- From:
Tumor
2017;37(4):334-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of tripartite motif 46 (TRIM46) on the proliferation and invasion of colorectal cancer cells and study its possible mechanism. Methods: After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expressions of TRIM46 mRNA and protein were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. CCK-8 assay and Transwell chamber assay were implemented to observe the effect of TRIM46 down-regulation on the proliferation and invasion of HCT116 cells, and the expression of Src homology 2 (SH2)-containing tyrosine phosphatase 1 (SHP1) was detected by Western blotting. Results: After TRIM46 siRNA was transfected into human colorectal cancer HCT116 cells, the expression levels of TRIM46 mRNA and protein were inhibited (both P < 0.05). Knockdown of TRIM 46 significantly suppressed the cell proliferation and invasion of HCT116 cells (both P < 0.05). However, the expression level of SHP1 was increased (P < 0.05). Conclusion: Ubiquitin ligase TRIM46 may improve the proliferation and invasion of human colorectal cancer cells through suppressing SHP1.
