Lignan 1 inhibits the proliferation of liver cancer HepG2 cells and induces apoptosis
10.3781/j.issn.1000-7431.2017.11.634
- Author:
Yingwen QI
1
Author Information
1. Department of General Surgery, First Hospital of Lanzhou University
- Publication Type:Journal Article
- Keywords:
Antineoplastic agents;
Caspase 3;
Lignan;
Liver neoplasms
- From:
Tumor
2017;37(2):143-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of lignan 1 on the proliferation and apoptosis of liver cancer HepG2 cells. Methods: The proliferation of HepG2 cells after treatment with different concentrations of lignan 1 was detected by MTT assay; the cell cycle distribution and the apoptosis were examined by FCM; the expression level of cleaved caspase 3 protein was determined by using Western blotting. Results: The proliferation of HepG2 cells after treatment with 1.25-20 μg/mL lignan 1 for 24, 48 and 72 h was inhibited in a time- and dose-dependent manner (all P < 0.05). The percentage of HepG2 cells in G2/M phase and the apoptotic rate of HepG2 cells after treatment with 2.5-20 μg/mL lignan 1 were increased (all P < 0.05). The expression level of cleaved caspase 3 protein was up-regulated (P < 0.05). Conclusion: Lignan 1 can inhibit the proliferation of liver cancer HepG2 cells and induce the apoptosis. This effect may be related to up-regulating the expression level of cleaved caspase 3 protein.
