SASH1-IQGAP1-E-cadherin signal cascade may regulate breast cancer metastasis
10.3781/j.issn.1000-7431.2017.33.088
- Author:
Shu LI
1
Author Information
1. Laboratory Medicine Department, Yongchuan Hospital, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Cadherins;
Gene expression regulation;
IQ motif-containing gtpase activating protein 1;
Sash1 protein
- From:
Tumor
2017;37(6):633-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the molecular mechanism of SAM-and SH3-domain containing 1 (SASH1) which serves as a novel tumor suppressor gene to regulate breast cancer metastasis. Methods: The expressions of SASH1, IQ motif-containing GTPase activating protein 1 (IQGAP1) and E-cadherin in breast cancer tissues were analyzed by immunohistochemistry (IHC). The correlation among SASH1, IQGAP1 and E-cadherin, as well as the association of SASH1 and IQGAP1 expressions with the clinical parameters of breast cancer patients were analyzed, respectively. The recombinant plasmids HAIQGAP1-pcDNA3.0 and pEGFP-C3-SASH1 were cloned and transfected into human embryonic kidney HEK-293T cells. The interaction of SASH1 and IQGAP1 was analyzed by immunoprecipitation-Western blotting. Results: In breast cancer tissues, there was a correlation between the expressions of SASH1 and IQGAP1 (P < 0.05), and the expressions of SASH1 and IQGAP1 proteins were respectively correlated with the expression of E-cadherin (both P < 0.001). In addition, the expressions of SASH1 and IQGAP1 proteins were correlated with tumor diameter and tumor grade (all P < 0.05), but without lymph node metastasis (both P > 0.05). The recombinant plasmids HAIQGAP1-pcDNA3.0 and pEGFP-C3-SASH1 were constructed successfully. After these recombinant plasmids were transfected into HEK-293T cells, the interaction between SASH1 and IQGAP1 was found. Conclusion: SASH1 interacts with IQGAP1, and which is closely related to the expression of E-cadherin. Therefore, it is suggested that SASH1 may form a new signaling cascade with IQGAP1 and E-cadherin to regulate breast cancer metastasis.
