Expression and function of VWCE gene in human breast cancer
10.3781/j.issn.1000-7431.2017.33.207
- Author:
Lili WAN
1
Author Information
1. Basic Medical College, Guangdong Medical University
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Cell migration assays;
Cell proliferation;
Gene expression;
von Willebrand factor;
VWCE
- From:
Tumor
2017;37(7):742-749
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the expression of von Willebrand factor Cand epidermal growth factor domains (VWCE ) gene in human breastcancer tissues, and to assess the effects of VWCE overexpression onproliferation and migration of breast cancer cells in vitro .Methods: The expression levels of VWCE mRNA and protein in 36pairs of human breast cancer tissues and their adjacent normal tissues as well as 5 kinds of breast cancer cell lines were detected by real-time fluorescentquantitative PCR and Western blotting, respectively. The recombinant lentivirus carryingVWCE gene was constructed and infected into breast cancer MCF-7 and SK-BR-3 cells. Afterthe overexpression of VWCE gene was verified by real-time fluorescent quantitative PCR andWestern blotting, the proliferation and migration of breast cancer cells were detected byCCK-8 and Transwell chamber assay, respectively.Results: The high expression rate of VWCE mRNA in breast cancer tissues was significantlylower than that in the corresponding adjacent tissues (22% vs 78%, P < 0.05), and wasrelated with the primary tumor stage (P < 0.05). The mean expression level of VWCE proteinin breast cancer tissues was lower than that in the corresponding adjacent tissues (P < 0.05).The expression levels of VWCE mRNA and protein in 5 kinds of breast cancer cell lines werelower than those in immortalized mammary epithelial cell line (all P < 0.05), respectively.After the breast cancer MCF-7 and SK-BR-3 cells were infected with VWCE overexpressionlentivirus, the expression of VWCE protein was significantly increased (both P < 0.05), whilethe cell proliferation and migration were significantly inhibited (all P < 0.05).Conclusion: VWCE is low expressed in human breast cancer tissues and cells. Overexpressionof VWCE can inhibit the proliferation and migration of breast cancer cells in vitro .
