Correlation of EpCAM expression in circulating tumor cells and colorectal tumor formation in nude mice
10.3781/j.issn.1000-7431.2017.11.421
- Author:
Qimei WANG
1
Author Information
1. Department of Oncology, Affiliated Hospital of Hunan Academy of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Circulating;
Colorectal neoplasms;
Epithelial cell adhesion molecule;
Mice;
Neoplastic cells;
Nude
- From:
Tumor
2017;37(8):840-845
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the colorectal tumor formation and metastasis of circulating tumor cells in nude mice. Methods: Human colorectal cancer HCT116-luc cells labeled with luciferase were implanted between the mucosa and the base of the cecum wall in nude mice. The circulating tumor cells in peripheral blood in nude mice were screened by epithelial cell adhesion molecule (EpCAM) immunomagnetic beads. The EpCAM-positive (EpCAM+) cells and the EpCAM-negative (EpCAM) cells were injected into the tail vein of the nude mice, respectively, then the tumor formation and the metastases in lung and liver in nude mice were observed. Results: The orthotopic transplanted tumor model of colorectal cancer in nude mice was successfully constructed. The tumor formation rate of EpCAM+circulating tumor cells in nude mice was 90%, the metastasis rates in lung and liver were 60% and 40%, respectively, and the metastasis rate in both liver and lung was 35%. There was no tumor formation of EpCAM circulating tumor cells in nude mice and also no metastasis occurred. The tumor formation rate and the metastasis rate between EpCAM+ and EpCAM groups had statistical differences (all P < 0.01). Conclusion: The tumor formation of EpCAM+ circulating tumor cells in nude mice is good, and the tumor prones to metastasis.
