Overexpression of brain-derived neurotrophic factor-antisense long non-coding RNA inhibits proliferation and invasion of hepatocellular carcinoma cells
10.3781/j.issn.1000-7431.2017.11.199
- Author:
Dawei GUO
1
Author Information
1. Fourth Department of General Surgery, Fourth Affiliated Hospital of China Medical University
- Publication Type:Journal Article
- Keywords:
Antisense;
Brain-derived neurotrophic factor;
Carcinoma;
Cell invasion;
Cell proliferation;
Hepatocellular;
Long non-coding;
RNA;
RNA
- From:
Tumor
2017;37(8):817-823
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of brain-derived neurotrophic factor-antisense long non-coding RNA (BDNF-AS) on proliferation and invasion of human hepatocellular carcinoma cells. Methods: The recombinant lentivirus carrying BDNF-AS gene was infected into hepatocellular carcinoma HepG2 and 97-H cells, respectively. The expression levels of BDNF-AS and BDNF mRNA were detected by reverse transcription PCR. The effects of BDNF-AS overexpression on the proliferation, apoptosis and invasion of HepG2 and 97-H cells were detected by EdU incorporation assay, Annexin V-FITC/propidium iodide (PI) double staining and Transwell chamber assay, respectively. Results: After infection with the recombinant lentivirus carrying BDNF-AS gene, the expression of BDNF-AS in hepatocellular carcinoma HepG2 and 97-H cells was significantly up-regulated (both P < 0.000 1), but the expression of BDNF mRNA in the two cells was significantly reduced (P < 0.000 1, P = 0.001). BDNF-AS overexpression had no effect on apoptosis of HepG2 and 97-H cells (both P > 0.05), but the proliferation and invasion abilities of the two cells were significantly decreased (P < 0.05, P = 0.001; both P < 0.000 1). Conclusion: BDNF-AS overexpression may play an important role in negatively regulating the growth and invasion of hepatocellular carcinoma cells.
