Down-regulation of SPOCK1 gene expression inhibits the proliferation, migration and invasion of bladder cancer cells
10.3781/j.issn.1000-7431.2017.11.262
- Author:
Changwen WU
1
Author Information
1. Department of Urology, Second Affiliated Hospital, Nanchang University
- Publication Type:Journal Article
- Keywords:
Cell movement;
Cell proliferation;
Gene, SPOCK 1;
RNA interference;
Urinary bladder neoplasms
- From:
Tumor
2017;37(9):936-943
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the expression of sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (SPOCK 1) gene in bladder cancer cells, and further to investigate the effects of down-regulation of SPOCK 1 gene expression on the proliferation, migration and invasion of bladder cancer cells. Methods: The expression level of SPOCK 1 gene in human ureteral epithelial immortalized cell line SV-HUC-1 and bladder cancer cell lines 5637 and T24 was detected by real-time fluorescent quantitative PCR (RFQ-PCR) and Western blotting, respectively. The siRNA fragments targeting SPCOK 1 gene were constructed and transfected into bladder cancer 5637 cells by liposome (as the experimental group); at the same time, the 5637 cells were transfected without anything as the blank control group and transfected with scramble siRNA as the negative control group. Then the expressions of SPOCK1 mRNA and protein in each group were detected by RFQ-PCR and Western blotting, respectively. The changes of proliferation, migration and invasion abilities of bladder cancer 5637 cells after SPOCK1 expression down-regulation were detected by CCK-8 method, Wound healing assay and Transwell chamber assay, respectively. Results: As compared with the ureteral epithelial immortalized cells, the expression levels of SPOCK1 mRNA and protein were higher in bladder cancer cells, especially in 5637 cells (both P < 0.05). After transfection with SPOCK1 siRNA, the expression of SPOCK 1 gene was downregulated (P < 0.05), the proliferation, migration and invasion of bladder cancer 5637 cells were significantly inhibited (all P < 0.05). Conclusion: SPOCK 1 gene is overexpressed in bladder cancer cells. The down-regulation of SPOCK 1 gene expression can reduce the proliferation, migration and invasion of bladder cancer 5637 cells.
