Expression of chromobox homolog 2 in colorectal cancer and its clinical significance
10.3781/j.issn.1000-7431.2017.33.271
- Author:
Ziyuan ZHU
1
Author Information
1. Department of Gastrointestinal Surgery, Third Affiliated Hospital of Guangzhou Medical University
- Publication Type:Journal Article
- Keywords:
Chromobox homolog 2;
Colorectal neoplasms;
Gene expression;
Prognosis
- From:
Tumor
2017;37(10):1056-1062
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of chromobox homolog 2 (CBX2) gene in colorectal cancer (CRC) tissues and cells, and to explore its clinical significance. Methods: The expressions of CBX2 mRNA and protein in CRC cells, normal colorectal epithelial cells, CRC tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The expression of CBX2 in CRC tissues and their corresponding adjacent normal tissues from 66 patients was detected by immunohistochemistry. The correlations of CBX2 expression with the clinicopathological features and prognosis of patients with CRC were analyzed. Results: The expressions of CBX2 mRNA and protein in CRC cells were higher than those in normal colonic epithelial FHC cells (all P < 0.05). The expression levels of CBX2 mRNA and protein in CRC tissues were also significantly higher than those in para-cancerous tissues (all P < 0.05). The positive rate of CBX2 expression in CRC tissues was significantly higher than that in adjacent normal tissues (53.0% vs 7.6%, P < 0.05). The expression of CBX2 was associated with TNM stage, lymph node metastasis, distant metastasis, and the survival status (all P < 0.05). The overall survival time of CRC patients with high expression of CBX2 was shorter than that of CRC patients with low expression of CBX2 (P = 0.01). Both CBX2 expression and distant metastasis were independent prognostic factors in patients with CRC (both P < 0.05). Conclusion: CBX2 is overexpressed in CRC tissues and cells, and is partly involved in the occurrence and development of CRC. Moreover, the expression of CBX2 is an independent prognostic factor for the patients with CRC.
